This research aims to reveal the construction of the anti-hero phenomenon in the genre of superhero-themed film from the perspective of transmedia storytelling. The Deadpool story universe, which constitutes the research universe, contains elements specific to an anti-hero character that develops integrated into many media. Qualitative and quantitative content analysis method is used for the methodology of the research. As a result of the analysis, it was determined that the transmedia storytelling approach was applied in the character-building of the anti-hero Deadpool. When the development processes of the media are examined in the research, it is seen that the Deadpool transmedia ecosystem consists of 8 stages. In the development of this ecosystem, size changes have been determined in terms of the structure of the medium and the quality of the content produced.